Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

Skip to main content

Table 1 Primers and probes in RT-qPCR

From: Low-fat diet, and medium-fat diets containing coconut oil and soybean oil exert different metabolic effects in untrained and treadmill-trained mice

Gene name

Sequence (5′ to 3′)

Universal Probe No.

Accession No.

PPARγ Coactivator 1α, (Pgc1a)

F: tgtggaactctctggaactgc

63

NM_008904.2

R: agggttatcttggttggcttta

PPARα, (Ppara)

F: ccgagggctctgtcatca

11

NM_011144.6

R: gggcagctgactgaggaa

PPARβ/δ, (Pparb/d)

F: atgggggaccagaacacac

11

NM_011145.3

R: ggaggaattctgggagaggt

ERRα, (Erra)

F: gtgggcatgctcaaggag

29

NM_007953.2

R: ggaaaggcaaagggtcca

ERRβ, (Errb)

F: ggcgttcttcaagagaacca

49

NM_011934.4

R: tccgtttggtgatctcacatt

ERRγ, (Errg)

F: aagtgggcatgctgaaagaa

29

NM_011935.3

R: cagcatctattctgcgcttg

Lipoprotein lipase, (Lpl)

F: tggataagcgactcctacttcag

22

NM_008509.2

R: tccctagcacagaagatgacc

Carnitine palmitoyltransferase 1B, (Cpt1b)

F: ccatcattgggcacctct

104

NM_009948.2

R: gtctccgtgtagcccaggt

Glucose transporter, 4 (Glut4)

F: tcgtcattggcattctggt

104

NM_009204.2

R: agcagtggccacagggta

Fatty acid transport protein, 1 (Fatp1)

F: cttcctaaggctgccattgt

49

NM_011977.3

R: ggcagtcatagagcacatcg

Myosin heavy chain, 2a (Myh2)

F: tcttctctggggcacaaact

22

NM_001039545.2

R: cccttcttcttggcaccttt

Carnitine palmitoyltransferase, 1A (Cpt1a)

F: aaagcaccagcacctgtacc

34

NM_013495.2

R: aacctccatggctcagacag

3-Hydroxy-3-methylglutaryl-CoA synthase, 2 (Hmgcs2)

F: ctgtggcaatgctgatcg

93

NM_008256.4

R: tccatgtgagttcccctca

Hypoxanthine-guanine phosphoribosyl transferase, (Hprt)

F: cctcctcagaccgcttttt

95

NM_013556.2

R: aacctggttcatcatcgctaa

  1. F: forward
  2. R: reverse